Journal: Journal of Functional Foods

Article Title: Egg white ovomucin hydrolysate inhibits intestinal integrity damage in LPS-treated Caco-2 cells

doi: 10.1016/j.jff.2021.104822

Figure Lengend Snippet: Fig. 4. Abundance changes of tight junction proteins in differentiated Caco-2 cells treated with ovomucin-protex 26L hydrolysate (1.0 mg/mL) for 24 h and LPS (20 μg/mL) for 2 h. Whole cell lysates were used for Western blot analysis of claudin-2 (A), claudin-3 (B), occludin (C), and ZO-1 (D), respectively. Band densities were quantified using Image Studio software (LI-COR Biosciences). The data are expressed as mean ± SEM of 4 independent experiments. Con means control group. *p < .05, **p < .01, significantly different from control group. #p < .05, significantly different from the LPS group.

Article Snippet: Rabbit monoclonal primary antibodies against claudin-2, claudin-3, occludin, ZO-1, phospho-NF-κB p65 (Ser536), NF-κB p65, phospho-p44/42 MAPK (ERK1/2) (Thr202/Try204), and p44/42 MAPK (ERK1/2) were obtained from Cell Signaling Technology (Whitby, ON, Canada).

Techniques: Western Blot, Software, Control

Journal: Journal of Functional Foods

Article Title: Egg white ovomucin hydrolysate inhibits intestinal integrity damage in LPS-treated Caco-2 cells

doi: 10.1016/j.jff.2021.104822

Figure Lengend Snippet: Fig. 5. Cellular distribution of tight junction proteins. Caco-2 cells were cultured on the coverslips for 21 d to undergo differentiation and treated with ovomucin- protex 26L hydrolysate for 24 h followed by LPS (20 μg/mL) for 2 h. After treatment, cells were fixed and stained with primary anti-claudin-2/3, anti-occludin, and anti-ZO-1 antibodies at 4 ℃ overnight. Anti-rabbit CFTM 488A antibody (Sigma) was used to visualize the localization of tight junction proteins by a CSU10 spinning disk confocal microscope (Quorum Technologies, Canada). Con means control group.

Article Snippet: Rabbit monoclonal primary antibodies against claudin-2, claudin-3, occludin, ZO-1, phospho-NF-κB p65 (Ser536), NF-κB p65, phospho-p44/42 MAPK (ERK1/2) (Thr202/Try204), and p44/42 MAPK (ERK1/2) were obtained from Cell Signaling Technology (Whitby, ON, Canada).

Techniques: Cell Culture, Staining, Microscopy, Control

Journal: The International Journal of Neuropsychopharmacology

Article Title: Activation of signaling pathways downstream of the brain-derived neurotrophic factor receptor, TrkB, in the rat brain by vagal nerve stimulation and antidepressant drugs

doi: 10.1017/s1461145713000977

Figure Lengend Snippet: Fig. 6. Western blot analysis of hippocampus CREB (≈50 kDa) phosphorylation at S133 after chronic 14 d VNS, sertraline (7.5 mg/ kg/d i.p.) or DMI (10 mg/kg/d i.p.). *p<0.01, Student’s t-test. The number of rats in each group is shown in the bars.

Article Snippet: Membranes were incubated at 4 °C overnight with the following primary antibodies: anti-pY515 (1:1000 in 2.5% BSA in TBST, Abcam, USA), anti-pY705 TrkB (1:1000 in 2.5% BSA in TBST, Abcam, USA), anti-pY816 TrkB (1:4000 in 2.5% BSA in TBST, Abcam, USA), and anti-TrkB (full length, 1:10000 in 2.5% BSA in TBST, Neuromics, USA), anti-PLCγ1 (1:1000), anti-PLC γ1 Y783 (1:250 in 5% BSA in TBST, Cell Signaling Technology, Inc, USA), anti-pERK T202/Y204 (1:1000 in 5% BSA in TBST, Cell Signaling Technology, Inc, USA), anti-ERK (1:2000 in 5% BSA in TBST, Cell Signaling Technology, Inc, USA), anti-AKT (1:1000 in 5% BSA in TBST, Cell Signaling Technology, Inc, USA), anti-pAKT T308 (1:1000 in 5% BSA in TBST, Cell Signaling Technology, Inc, USA), anti-CREB (1:2000 in 5% BSA in TBST, Cell Signaling Technology, Inc, USA), anti-pCREB S133 (1:1000 in 5% BSA in TBST, Cell Signaling Technology, Inc, USA), anti- pp70 S6 kinase T389 (1:1000 in 5% BSA in TBST, Cell Signaling Technology, Inc, USA) and anti-p70 S6 kinase (1:1000 in 5% BSA in TBST, Cell Signaling Technology, Inc, USA).

Techniques: Western Blot, Phospho-proteomics